The Protein Microarray Analyser software presented here includes the following tools: (1) neighbourhood background correction, (2) net intensity correction, (3) user-defined noise threshold, (4) user-defined CV threshold amongst replicates and (5) assay controls, (6) composite ‘pin-to-pin’ normalization amongst sub-arrays, and (7) ‘array-to-array’ normalization amongst whole arrays.

Features

  • Neighbourhood background correction
  • Net intensity correction
  • User-defined noise threshold
  • User-defined CV threshold amongst replicates and assay controls
  • Composite ‘pin-to-pin’ and ‘array-to-array’ normalization

Project Samples

Schematic of the Protein Microarray Analyser data processing and normalization pipeline. Extracted raw data is corrected and filtered to remove or flag problematic data and obtain high quality results that can then be used across a multitude of appropriate data analysis tools Protein Microarray Analyser software interface. This user-friendly interface allows the user to select raw data files, select default or custom settings, and lists the methods to be run on the dataset

Project Activity

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