123FASTQ performs all the pre-processes of Illumina next-generation sequencing reads (FASTQ files) easier than ever. 

Download the quick user manual for the latest version:
https://dl.adbioinformatics.net/NGSNeeds/myTools/123Fastq_v1.3_Manual.pdf

Authors:
Milad Eidi, Samaneh Abdolalizadeh, Mohammad Hossein Nassirpour

Supervisors:
Javad Zahiri, PhD
University of California San Diego 

Masoud Garshasbi, PhD
Tarbiat Modares University, Tehran, Iran


If you use 123FASTQ, please cite this preprint:
123FASTQ: an intuitive and efficient tool for preprocessing Illumina FASTQ reads
https://www.biorxiv.org/content/10.1101/2024.03.08.584032v1


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Take care of the details and ensure you use the latest version.
We hope this helps...

Features

  • Next generation sequencing reads quality check
  • Quality check of paired-end reads
  • Flexible trimmer
  • Trim recommendations based on quality check results
  • Adapter decontamination by two approaches
  • Fast5 to FASTQ conversion
  • SAM/BAM to FASTQ conversion
  • Cross platform (unix, windows)
  • Written in java

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