Aquileo | Recent changes to Project FCSalyzer

Aquileo | Overlay Problem

Sat, 29 Nov 2025 19:56:30 -0000

Hi Beel,

the labels disappear if there is more than one possible label.

So on the y-axis the label disappears in overlays that are scaled to maximum (like in your example) because the different graphs that are overlaid have different event counts to reach this maximum. Take a look at the single plots - which event counts are shown? If one plot has 10 000 events on the y-axos, the second has 8 000 and the third has 9 000, then there is no "correct" event count to use, so it disappears. If the histograms are scaled to the same fixed number, then the y-axis will show the event count.

the same is true for the x-axis. In your example, the bottom plot shows "APC", which will happen when all three overlays show APC. In the top plot, the x-axis is unlabeled, so the three histograms show not the same parameter (for example FITC, APC, APC), so there is no "correct" x-label and it disappears. This could also happen (to be honest I am not sure right now) if it is the same parameter, but with different descriptions (like "CD4 APC", "CD4 APC", "CD8 APC").

I hope that clarifies the situation. Take a look at your plots please to see if my explanation fits your situation. If not, then there might be a bug. let me know!

Thanks,
Sven

Aquileo | Overlay Problem

Sat, 29 Nov 2025 14:17:18 -0000

Why does the scale of the Y-axis disappear when I want to do the overlay? And also for the X-axis, the title 'PE-A' suddenly disappeared when doing the overlay (The blue one, upper), unlike the APC one below (red). Any solution for these problems? Thank you.

Aquileo | I can't open my FCS files

Sat, 10 May 2025 20:25:01 -0000

Hi,
I just tried it on my computer with the latest version 0.9.22 and it works for me.
Please see the attached image, which shows the dialog as it looks for me.
So right now I can't say what the problem might be. Can you please describe it in more detail, and possible add a screenshot and example data?
Thanks,
Svev

Aquileo | I can't open my FCS files

Sat, 10 May 2025 13:25:42 -0000

hello, could someone help me with deriving my parameter? i cant divide its not giving me 2 boxen but only one

Aquileo | I can't open my FCS files

Thu, 27 Feb 2025 19:21:47 -0000

FCX files are FCSalyzers internal documents for analysis. Into these documents, plots are based that display the data of selected FCS files.
Please read the Wiki and watch the tutorial videos: https://youtu.be/h8LTHK26lHM

Aquileo | I can't open my FCS files

Thu, 27 Feb 2025 08:55:26 -0000

Hello, does anyone can help me? I can't open my FCS files even though the option is FCS document. It only allowed for FCX files.

Aquileo | Way to include keyword/annotation in statistics?

Thu, 23 May 2024 18:22:35 -0000

Hi Scott,

unfortunately that is not currently possible (neither of the two options you suggested). I can put in on my ToDo, but as you can see from the releases, I somehow never get around to actually implement changes.
Sorry and I hope that you can still get some use out of FCSalyzer.

Best regards,
Sven

Aquileo | Way to include keyword/annotation in statistics?

Thu, 23 May 2024 13:35:36 -0000

Our machine's software (guava incyte) puts the sample decriptions inside the keyword GTI$SAMPLEID. Is there a way to set this as our "sample descr" to be used in statistics for plots? Or, alternatively, is there a way to include this extra keyword with statistics? (We can put the keyword in annotations, which is nice, but we want it also associated with our statistics).

Aquileo | setting the axis values

Thu, 18 Apr 2024 20:10:01 -0000

Dear Giovanna,

I don't quite understand the question. What do you mean with " some ive results in mean or median calculation"?

Thanks,
Sven

Aquileo | setting the axis values

Thu, 18 Apr 2024 14:05:18 -0000

Hi I am Giovanna and we are tryng to use FCSalyzer for BioRad S3 data. with biparametric fluorescence data (Green and Red, ie JC1 fluorescence or DiClorofluorescein /PI fluorescences) when we perform statistic, we found some ive results in mean or median calculation. We don't undersatand why and How it is possible...I wait for your response, my best regards
Giovanna