The name "MFEprimer" comes from the title of the first paper (2009, Bioinformatics): Multiple Factor Evaluation of the specificity of PCR primers. And both the version 1.0 and version 2.0 (2012, NAR) are only focused on primer specificty, leaving dimers and hairpins for users. However, dimers and hairpins are very important factors affecting success of PCR. So, for version 3.0, we expand the functions of MFEprimer, and make it as a full-functional primer quality control (PrimerQC, maybe the new name) program.

1. genomewide specificity check;
2. new and fast dimer check algorithm;
3. new and fast hairpin check algorithm;
4. primers compatible check for multiplex PCR;
5. thermodynamics based hybridization analysis;
6. core algorithm was rewritten with golang, which is fast and parallel.

1. Checking quality of primers against 16S databases (e.g., Bacteria 16S, Fungal 28S and Archaea 16S) will consume tens of minutes, even for hours, please be patient. You may refresh the page later to check the result.
2. Register an account to help you manage your tasks, you won't miss or forget any tasks any more.

1. iGeneTech Bioscience Co., Ltd.
2. Xiaolei Wang & Dr. Dongsheng Zhao