LoTempPCR^*  with HiFiDNA polymerase 

unloading the power of automated Sanger sequencing

in DNA testing for Pap cytology specimens

Perform DNA sequencing for HPV genotyping beyond a reasonable doubt -  BMC Clin Path 2009, 9:3 (22 May 2009).   

No-false positive Lyme disease DNA testing;

Validate the DNA of Neisseria gonorrhoeae opa genes beyond a reasonable doubt;

Validate the DNA of Chlamydia trachomatis cryptic plasmid beyond a reasonable doubt;

Using the GenBank database as the standard for validating sequence algorithms;

On 5% of a liquid-based Pap cytology specimen without need of DNA extraction.

These are cutting-edge molecular tests developed by pathologists/ cytotechnologists for pathologists/cytotechnologists to serve gynecologists and their patients at sustainable cost.  

Application of LoTemp PCR/DNA sequencing recent publications:

1. Detection of human papillomavirus L1 gene DNA fragments in postmortem blood and spleen after Gardasil vaccination-A case report

2. Detection of human papillomavirus (HPV) L1 gene DNA possibly bound to particulate aluminum adjuvant in the HPV vaccine Gardasil

3. Guidelines for the use of molecular tests for the detection and genotyping of human papillomavirus from clinical specimens

4. Early Lyme disease with spirochetemia - diagnosed by DNA sequencing.

* Lee SH, Vigliotti VS, Vigliotti JS, Pappu S, Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories. Infect Agent Cancer 2007:2:11.

HiFiDNA.com 2006-2013